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Nitrogen: the double-edged sword

Nitrogen is a component of protein and DNA and, as such, is essential to all living things. Prior to the Industrial Revolution, around 97% of the nitrogen supporting life on earth was fixed biologically. Over the last century, intensification of farming, coupled with a lack of understanding of soil microbial communities, has resulted in reduced biological activity and an increased application of industrially produced forms of nitrogen to agricultural land.

Globally, over $100 billion of nitrogen fertilizers are applied to crops and pastures every year. Between 10 and 40% of the applied N is taken up by plants. The other 60-90% is leached into water, volatilized into the air or immobilized in soil.

Impacts of inorganic nitrogen

The application of high rates of inorganic nitrogen in agricultural systems has had many unintended negative consequences for soil function and environmental health. Data from North America’s longest running field experiment on the impacts of farm production methods on soil quality have revealed that high nitrogen inputs deplete soil carbon, impair soil water-holding capacity – and, ironically, also deplete soil N (Khan et al. 2007, Larson 2007).

Taken together, these factors have been implicated as the underlying cause of widespread reports of yield stagnation around the world (Mulvaney et al. 2009).

The evidence suggests that although nitrogen is essential to plant growth, the application of large amounts of N as inorganic fertilizer is detrimental to soil — and also detrimental to water. The USDA estimates that the cost of removing nitrate from U.S. drinking water is more than $4.8 billion per year, while nitrogen run-off from farmland is the single largest source of nutrient pollution contributing to the massive ‘dead zone’ in the Gulf of Mexico (Ceres 2014).

Fortunately, the news is not all bad. Rates of fertilizer application have decreased in recent years in some developed countries. France, Germany, and the United Kingdom have achieved success in this area, maintaining high yields with forty to fifty percent less fertilizer than used in the 1980s (Krietsch 2014).

Cost-effective nitrogen management is the key to profitable and productive farming. It is also the key to building soil carbon. Stable forms of soil carbon (such as humus) cannot form in the presence of high levels of inorganic nitrogen, due to the inhibition of the microbes essential to sequestration.

Biological nitrogen fixation (BNF)

On a global scale, biological nitrogen fixation accounts for around 65% of the nitrogen used by crops and pastures. There is scope for considerable increase. The supply of nitrogen is inexhaustible, as dinitrogen (N2) comprises almost 80% of the earth’s atmosphere. The key is to transform inert nitrogen gas to a biologically active form.

Much of the nitrogen currently used in agriculture derives from the Haber-Bosch process, developed in the early 1900s. This process catalytically combines atmospheric nitrogen with hydrogen derived from natural gas or coal, to produce ammonia under conditions of high temperature and pressure. The Haber-Bosch process uses non-renewable resources, is energy intensive and expensive.

Fortunately – thanks to some ‘enzymatic magic’ – atmospheric nitrogen can be transformed to ammonia by a wide variety of nitrogen-fixing bacteria and archaea – for free.

Ideally, newly fixed ammonia is rapidly incorporated into organic molecules such as amino acids and humus. These stable molecules are vital to soil fertility and cannot be volatilized or leached from the soil system. Importantly, the stabilization of nitrogen requires a steady supply of carbon – also fixed biologically. We’ll come to that in a moment.

Which microbes are involved?

It is important to recognize that the ability to fix nitrogen is not limited to bacteria associated with legumes. Chlorophyll is part of a protein complex – hence wherever you see green plants – there will also be an association with nitrogen-fixing bacteria or archaea.

Unlike rhizobial bacteria, most nitrogen-fixing microbes are not able to be cultured in the laboratory. This has presented technical challenges to assessing their ecological function. Recent bio-molecular methods for determining the presence of nifH, the gene for nitrogenase reductase, have revealed a dizzying array of free-living and associative nitrogen-fixing bacteria and archaea across a wide range of environments.

Although procedures for quantifying the amount of nitrogen fixed by many of these groups are lacking, what we do know is that the diversity and abundance of nitrogen fixing microbes are much greater where there is living groundcover (particularly plants in the grass family) throughout the year, compared to soils that have been bare fallowed.

In addition to nitrogen-fixing bacteria and archaea, mycorrhizal fungi are also vitally important to the N-fixing process. Although mycorrhizal fungi do not fix nitrogen, they transfer energy, in the form of liquid carbon (Jones 2008) to associative nitrogen fixers. They also transport biologically fixed nitrogen to plants in organic form, for example, as amino acids, including glycine, arginine, chitosan and glutamine (Leake et al. 2004, Whiteside et al. 2009).

The acquisition and transfer of organic nitrogen by mycorrhizal fungi is highly energy efficient. This pathway closes the nitrogen loop, reducing nitrification, denitrification, volatilization and leaching. Additionally, the storage of nitrogen in the organic form prevents soil acidification.

The liquid carbon pathway

Despite its abundance in the atmosphere, nitrogen is frequently the most limiting element for plants. There is a reason for this. Carbon, essential to photosynthesis and soil function, occurs as a trace gas, carbon dioxide, currently comprising 0.04% of the atmosphere. The most efficient way to transform CO2 to stable organic soil complexes (containing both C and N) is via the liquid carbon pathway. The requirement for biologically-fixed nitrogen drives this process.

If plants were able to access nitrogen directly from the atmosphere, their growth would be impeded by the absence of carbon-rich topsoil. We are witnessing an analogous situation in agriculture today. When inorganic nitrogen is provided, the supply of carbon to associative nitrogen fixing microbes is inhibited, resulting in carbon-depleted soils.

Reduced carbon flows impact a vast network of microbial communities, restricting the availability of essential minerals, trace elements, vitamins and hormones required for plant tolerance to environmental stresses such as frost and drought and resistance to insects and disease. Lowered micronutrient densities in plants also translate to reduced nutritional value of food.

Above ground, plant growth often appears ‘normal’, hence the connection to failing soil function may not be immediately obvious. But underneath, our soils are being destroyed.

Ideally, land management practices – and any amendments used in agriculture – should enhance photosynthetic rate and increase the flow of carbon to soil, by supporting plant-associated microbial communities.

figure1photo Jill Clapperton

Fig. 1. Cross section of a plant root showing the thread-like hyphae of mycorrhizal fungi. Mycorrhiza deliver sunlight energy, packaged as liquid carbon, to a vast array of soil microbes involved in plant nutrition and disease suppression. Organic nitrogen, phosphorus, sulphur, potassium, calcium, magnesium, iron and essential trace elements such as zinc, manganese and copper are returned to plant hosts in exchange for carbon. Nutrient transfers are inhibited when high rates of inorganic nitrogen and/or inorganic phosphorus are applied.

Determining brix levels with a refractometer is an easy way to assess the rate at which green leaves are photosynthesizing and hence supporting associative soil microbes. Anything that reduces the photosynthetic capacity of land or the photosynthetic rate of vegetation is NOT sustainable.

How can we utilize our understanding of the liquid carbon pathway to restore natural fertility to agricultural land?

Aggregation is the key

Aggregates are the small ‘lumps’ in soil that provide tilth, porosity and water-holding capacity. Unless soils are actively aggregating, they will not be fixing significant amounts of atmospheric N or sequestering stable forms of carbon. All three functions (aggregation, biological N-fixation and stable C-sequestration) are inter-dependent.

The microbes involved in the formation of soil aggregates require an energy source. This energy initially comes from the sun. In the miracle of photosynthesis, green plants transform light energy, water and carbon dioxide into biochemical energy, which is transferred to soil as liquid carbon via an intricate network of mycorrhizal fungi and associated bacteria.

What do soil aggregates look like?

Fig. 2. The 2 wheat plants on the left were grown with perennial grasses in a Pasture Crop treatment while the wheat plant on the right was grown in adjacent bare soil, amended with 100kg/ha DAP.

Fig. 2. The 2 wheat plants on the left were grown with perennial grasses in a Pasture Crop treatment while the wheat plant on the right was grown in adjacent bare soil, amended with 100kg/ha DAP.

Note the little lumps adhering to the roots of the Pasture Cropped wheat (Fig. 2). These clusters are formed by microbes utilizing liquid carbon from  the roots. Microaggregates, too small to be seen with the naked eye, are bound together by microbial glues and gums and the hyphae of mycorrhizal fungi (also using liquid carbon), to form bigger lumps called macroaggregates, generally 2-5mm in size (around 1/8th of an inch in non-metric terms).

Macroaggregates are essential to soil tilth, structure, aeration, infiltration, water-holding capacity, biological nitrogen fixation and carbon sequestration. In short, it is not possible to maintain healthy soils without them.

Let’s take a look inside a macroaggregate, courtesy of this fabulous illustration (Fig. 3) by Rudy Garcia, State Agronomist with the USDA Natural Resources Conservation Service in New Mexico.
A key feature is that moisture and liquid carbon levels are higher within root-supported aggregates than in the surrounding soil, while the partial pressure of oxygen is lower within root-supported aggregates than in the surrounding soil. These conditions are essential for the functioning of the nitrogenase enzyme utilized for biological nitrogen fixation and also to the formation of humus.

Fig. 3. Diagrammatic representation of a soil macroaggregate. The green vertical line is a fine feeder root and the green horizontal lines are root hairs. The assortment of red and orange particles are microaggregates while the scattered brown shapes represent particulate organic matter. Light colored spheres are sand grains of various sizes, often coated with oxides of iron and aluminum, while the small yellow ellipses are bacterial colonies, including nitrogen-fixing and phosphorus solubilizing species. The fine strands running in multiple directions are the hyphae of mycorrhizal fungi, essential to the enmeshing of the soil particles and the supply of carbon to microbial communities within the aggregate. Depending on soil pH, there will also be precipitated minerals such as iron phosphate or calcium phosphate. The blue background is water held within the aggregate.

Fig. 3. Diagrammatic representation of a soil macroaggregate. The green vertical line is a fine feeder root and the green horizontal lines are root hairs. The assortment of red and orange particles are microaggregates while the scattered brown shapes represent particulate organic matter. Light colored spheres are sand grains of various sizes, often coated with oxides of iron and aluminum, while the small yellow ellipses are bacterial colonies, including nitrogen-fixing and phosphorus solubilizing species. The fine strands running in multiple directions are the hyphae of mycorrhizal fungi, essential to the enmeshing of the soil particles and the supply of carbon to microbial communities within the aggregate. Depending on soil pH, there will also be precipitated minerals such as iron phosphate or calcium phosphate. The blue background is water held within the aggregate.

Within root-supported aggregates, liquid carbon is transferred from fine root hairs to the hyphae of mycorrhizal fungi, thence to highly complex microbial communities. The microbes receiving this carbon – and their metabolites – are instrumental in the transformation of simple sugars to highly stable humic polymers, a portion of which comprises biologically fixed nitrogen and bacterially-solubilized phosphorus. Iron and aluminum, which occur as oxides in the mineral matrix, are important catalysts.

It is now recognized that plant root exudates make a greater contribution to stable forms of soil carbon (that is, to organo-mineral complexes containing organic carbon and organic nitrogen) than does the above-ground biomass (Schmidt et al. 2011)

But here’s the rub. Mycorrhizal colonization is low when large quantities of inorganic N are applied … and mycorrhiza are inactive when plants are absent. Hence biological nitrogen fixation and humification are rare in agricultural systems where heavily N-fertilized crops are rotated with bare fallows. Further, it has been shown that up to 80kgN/ha can be volatilized from bare summer fallows due to denitrification. If green plants are present, this N can be taken up and recycled, preventing irretrievable loss.

When soil is bare there is no photosynthesis and very little biological activity. Bare soils lose carbon and nitrogen, nutrient cycles become dysfunctional, aggregates deteriorate, structure declines and water-holding capacity is reduced. Bare fallows, designed to store moisture and retain nutrients, become self-defeating.

The maintenance of bare fallows – or the use of high rates of inorganic N in crops or pastures –
or worse, both – results in the uncoupling of the nitrogen and carbon cycles that have functioned synergistically for millennia. Photosynthesis is the most important process underpinning life on earth. Non-legume biological nitrogen fixation is the second.

It is important to distinguish between the nitrogen fixed within nodules on the roots of leguminous plants and the nitrogen fixed within aggregates formed in association with the roots of non-legumes. In the latter, the nitrogen can be incorporated into amino acids and humic substances. This is much less likely to occur in stands of pure legume. Legumes are high in minerals and trace elements and form an important part of agricultural systems. However, unless legumes are grown in mixtures with non-legumes, they can deplete soil carbon via the same mechanism as it is depleted by high-analysis fertilizer.

Enhancing the liquid carbon pathway

There is increasing recognition of the fundamental importance of soil microbial communities to plant productivity. Unfortunately, many biological functions are compromised by commonly used agricultural practices.

Redesign of farming practice is not difficult. The first step is recognition of the importance of the year-round presence of green plants and the microbial populations they support.

Redesign has the potential to significantly reduce the impact of many ‘problems’ associated with chemical farming, including loss of soil C, reduced soil N, soil compaction, declining pH, low nutrient availability, herbicide resistance and impaired water-holding capacity.

There are four basic principles for regenerative agriculture, proven to restore soil health and increase levels of organic carbon and nitrogen. From these, landholders can build an integrated land management package that suits their individual property and paddock needs.
1) The first principle is the maintenance of year-round living cover, via perennial pastures on grazed land and/or multi-species cover crops on farmed land. Almost every living thing in and on the soil depends on green plants (or what was once a green plant) for its existence. The more green plants, the more life.

It’s well accepted that groundcover buffers soil temperatures and reduces erosion, but it is perhaps less recognized that actively growing green groundcover also fuels the liquid carbon pathway which in turn supports, among other things, mycorrhizal fungi, associative N-fixing bacteria and phosphorus-solubilizing bacteria – all of which are essential to both crop nutrition and the formation of stable humified carbon.

2) The second principle is to provide support for the microbial bridge, to enhance the flow of carbon from plants to soil. This requires reducing inputs of high analysis N & P fertilizers that inhibit the

Conclusion

Biological nitrogen fixation is the key driver of the nitrogen and carbon cycles in all natural ecosystems, both on land and in water. When managed appropriately, biological nitrogen fixation can also be the major determinant of the productivity of agricultural land.

Many farmers around the world are discovering first-hand how the change from bare fallows to biodiverse year-long green plant cover, coupled with appropriate livestock management and reduced applications of inorganic nitrogen, can restore natural topsoil fertility.

Improving soil function delivers benefits both on-farm and to the wider environment.

For further information, visit www.amazingcarbon.com

Literature cited

Ceres (2014). Water and climate risks facing U.S. corn production. 11 June 2014. http://www.ceres.org/issues/water/agriculture/the-cost-of-corn/the-cost-of-corn

Jones, C.E. (2008). Liquid carbon pathway unrecognized. Australian Farm Journal, July 2008, pp.15-17. www.amazingcarbon.com

Khan, S.A, Mulvaney, R.L, Ellsworth, T.R. and Boast, C.W. (2007). The myth of nitrogen fertilization for soil carbon sequestration. Journal of Environmental Quality 36:1821-1832. doi:10.2134/jeq2007.0099

Krietsch, B (2014). Artificial fertilizer use levels-off as regions reach state of diminishing returns. http://foodtank.com/news/2014/04/fertilizer-use-levels-off-as-regions-reach-state-of-diminishing-returns

Larson, D. L (2007). Study reveals that nitrogen fertilizers deplete soil organic carbon. University of Illinois news, October 29, 2007. http://www.aces.uiuc.edu/news/internal/preview.cfm?NID=4185

Leake, J.R., Johnson, D., Donnelly, D.P., Muckle, G.E., Boddy, L. and Read, D.J. (2004). Networks of power and influence: the role of mycorrhizal mycelium in controlling plant communities and agroecosystem functioning. Canadian Journal of Botany, 82: 1016-1045. doi:10.1139/B04-060

Mulvaney, R.L, Khan S.A. and Ellsworth, T.R. (2009). Synthetic nitrogen fertilizers deplete soil nitrogen: a global dilemma for sustainable cereal production. Journal of Environmental Quality 38: 2295-2314. doi:10.2134/jeq2008.0527

Schmidt, M. W. I., Torn, M. S., Abiven, S., Dittmar, T., Guggenberger, G., Janssens, I. A., Kleber, M., gel-Knabner, I. K., Lehmann, J., Manning, D. A. C., Nannipieri, P., Rasse, D. P.,  Weiner, S. and Trumbore, S. E. (2011). Persistence of soil organic matter as an ecosystem property. Nature, 478: 49-56. doi:10.1038/nature10386

Whiteside, M. D., Treseder, K. K. and Atsatt, P. R. (2009). The brighter side of soils: Quantum dots track organic nitrogen through fungi and plants. Ecology 90:100–108.   doi:10.1890/07-2115.1